Hotspot

rpoA-rps11

LSC · 78705-78775 · 71 bp

Back to Vuhuangia

Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACTTCCACTGTAGTGTCCGA	GGATGTAACCCCTATGCCAC	50.0	55.0	58.0	57.7	178 bp	1.0	78.2
rpoA-rps11_p2	TTCCACTGTAGTGTCCGAGT	GGATGTAACCCCTATGCCAC	50.0	55.0	58.0	57.7	176 bp	1.0	78.2
rpoA-rps11_p3	CACTTCCACTGTAGTGTCCG	GGATGTAACCCCTATGCCAC	55.0	55.0	57.9	57.7	179 bp	1.0	77.7
rpoA-rps11_p4	TTCCACTGTAGTGTCCGAGT	AATGGCTGTAGACCTCCGAA	50.0	50.0	58.0	58.4	154 bp	1.0	77.0
rpoA-rps11_p5	TTCCACTGTAGTGTCCGAGT	ATGGCTGTAGACCTCCGAAA	50.0	50.0	58.0	58.4	153 bp	1.0	77.0