Hotspot

petA-psbJ

LSC · 63152-64161 · 1010 bp

Back to Vuhuangia

Pi 0.000992

MarkerSeek score 59.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 1
pi: 0.000992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1065-1067 bp	1.0	74.9
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1064-1066 bp	1.0	74.4
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1064-1066 bp	1.0	74.0
petA-psbJ_p4	CGTGTCCAAGGTCTTTTGGT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.3	57.5	1143-1145 bp	1.0	73.7
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.7	57.4	1063-1065 bp	1.0	73.5