Hotspot

accD-psaI

LSC · 58762-59497 · 736 bp

Back to Vuhuangia

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	852 bp	1.0	79.1
accD-psaI_p2	CTCCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	848 bp	1.0	79.1
accD-psaI_p3	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	858 bp	1.0	78.5
accD-psaI_p4	CTCCACGCCTTCTTTCCTTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	854 bp	1.0	78.4
accD-psaI_p5	CGACTTAATCGTACCGCGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	903 bp	1.0	76.9