Hotspot

ndhG

SSC · 116080-116610 · 531 bp

Back to Vrydagzynea

Pi 0.0169

MarkerSeek score 52.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 9
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACTTCGGCTTGTGATCAGTC	TGTGAAGTATGTGTTCGCGT	50.0	45.0	57.9	57.9	1238-1259 bp	1.0	80.7
ndhG_p2	ACTTCGGCTTGTGATCAGTC	TCACATCAGAGCGTTTTCGT	50.0	45.0	57.9	57.9	1294-1315 bp	1.0	80.6
ndhG_p3	ACTTCGGCTTGTGATCAGTC	GTGTTCGCGTATGTCCCATA	50.0	50.0	57.9	57.8	1228-1249 bp	1.0	80.2
ndhG_p4	ACTTCGGCTTGTGATCAGTC	TATGTGTTCGCGTATGTCCC	50.0	50.0	57.9	57.8	1231-1252 bp	1.0	80.2
ndhG_p5	ACTTCGGCTTGTGATCAGTC	CACATCAGAGCGTTTTCGTG	50.0	50.0	57.9	57.8	1293-1314 bp	1.0	80.0