Hotspot

trnL-UAG-ccsA

SSC · 112052-112167 · 116 bp

Back to Vrydagzynea

Pi 0.0517

MarkerSeek score 67.50

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 6
pi: 0.0517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	891-893 bp	1.0	81.1
trnL-UAG-ccsA_p2	CTTTTTGACTGTCCGGTGGA	ACCTGATGTGGCAAAACCTT	50.0	45.0	58.0	57.9	1046-1048 bp	1.0	80.8
trnL-UAG-ccsA_p3	CTTTTTGACTGTCCGGTGGA	TTCCAATAAGAGCCCCATGC	50.0	50.0	58.0	57.9	1444-1446 bp	1.0	80.7
trnL-UAG-ccsA_p4	CTTTTTGACTGTCCGGTGGA	CTGCTCCAGAAAGAATGCCT	50.0	50.0	58.0	57.9	1410-1412 bp	1.0	80.3
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	TTCCAATAAGAGCCCCATGC	50.0	50.0	57.9	57.9	923-929 bp	1.0	79.5