Hotspot

ndhF-rpl32

SSC · 111251-111429 · 179 bp

Back to Vrydagzynea

Pi 0.0565

MarkerSeek score 77.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 179
variable_sites: 7
pi: 0.0565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACATAGTAACTGGGAGCGGA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	247-300 bp	1.0	84.5
ndhF-rpl32_p2	TAACTGGGAGCGGAAAAAGG	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	241-294 bp	1.0	84.2
ndhF-rpl32_p3	ACATAGTAACTGGGAGCGGA	CCACCGGACAGTCAAAAAGT	50.0	50.0	57.8	58.3	246-299 bp	1.0	82.2
ndhF-rpl32_p4	TAACTGGGAGCGGAAAAAGG	CCACCGGACAGTCAAAAAGT	50.0	50.0	57.8	58.3	240-293 bp	1.0	82.0
ndhF-rpl32_p5	GACATAGTAACTGGGAGCGG	TCCACCGGACAGTCAAAAAG	55.0	50.0	57.5	58.0	248-301 bp	1.0	81.6