Hotspot

trnF-GAA-ndhJ

LSC · 47371-47937 · 567 bp

Back to Vrydagzynea

Pi 0.0159

MarkerSeek score 67.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 567
variable_sites: 9
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AGAGTACTGGTCGGGATAGC	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	725 bp	1.0	79.8
trnF-GAA-ndhJ_p2	AGTACTGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	723 bp	1.0	79.8
trnF-GAA-ndhJ_p3	GAGTACTGGTCGGGATAGCT	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	724 bp	1.0	79.8
trnF-GAA-ndhJ_p4	TACTGGTCGGGATAGCTCAG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	721 bp	1.0	79.8
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	677 bp	1.0	79.6