Hotspot

rps4-trnT-UGU

LSC · 44940-45252 · 313 bp

Back to Vrydagzynea

Pi 0.0321

MarkerSeek score 60.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 10
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCAGCCGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	55.6	55.0	58.4	57.9	422-423 bp	1.0	61.8
rps4-trnT-UGU_p2	CCCAGCCGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	55.6	50.0	58.4	57.8	390-391 bp	1.0	61.1
rps4-trnT-UGU_p3	CCCAGCCGGCGTATTTTT	CTTATATCCGCCCGCTTAGC	55.6	55.0	58.4	58.4	441-442 bp	1.0	59.9
rps4-trnT-UGU_p4	CCCAGCCGGCGTATTTTT	ATTGCTCTTATATCCGCCCG	55.6	50.0	58.4	57.6	447-448 bp	1.0	59.7
rps4-trnT-UGU_p5	CCCAGCCGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	55.6	55.0	58.4	57.5	430-431 bp	1.0	58.8