Hotspot

psaA-ycf3

LSC · 40903-41600 · 698 bp

Back to Vrydagzynea

Pi 0.0089

MarkerSeek score 66.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 6
pi: 0.0089
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTGGCCCATTCTTCGAAAGA	ACAGGCTTGGTTCGATCAAG	50.0	50.0	57.9	58.2	880-893 bp	1.0	80.3
psaA-ycf3_p2	TCTGGCCCATTCTTCGAAAG	ACAGGCTTGGTTCGATCAAG	50.0	50.0	57.9	58.2	881-894 bp	1.0	80.3
psaA-ycf3_p3	CTGGCCCATTCTTCGAAAGA	GACAGGCTTGGTTCGATCAA	50.0	50.0	57.9	58.2	881-894 bp	1.0	80.3
psaA-ycf3_p4	TCTGGCCCATTCTTCGAAAG	GACAGGCTTGGTTCGATCAA	50.0	50.0	57.9	58.2	882-895 bp	1.0	80.3
psaA-ycf3_p5	CTGGCCCATTCTTCGAAAGA	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	836-849 bp	1.0	80.3