Hotspot

petN-psbM

LSC · 28313-28850 · 538 bp

Back to Vrydagzynea

Pi 0.0243

MarkerSeek score 59.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 13
pi: 0.0243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	ACGTCAAAACAGTCAGCCAA	55.0	45.0	58.1	58.3	592-642 bp	1.0	79.1
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	ACGTCAAAACAGTCAGCCAA	55.0	45.0	58.1	58.3	591-641 bp	1.0	79.1
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	589-639 bp	1.0	78.1
petN-psbM_p4	TGGGGAAGGAGTGGACTTTA	ACGTCAAAACAGTCAGCCAA	50.0	45.0	57.6	58.3	588-638 bp	1.0	76.6
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	TACGTCAAAACAGTCAGCCA	55.0	45.0	58.1	57.4	592-642 bp	1.0	76.3