Hotspot

trnS-GCU-trnG-GCC

LSC · 8065-8554 · 490 bp

Back to Vrydagzynea

Pi 0.0292
MarkerSeek score 65.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
490
variable_sites
14
pi
0.0292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 549-559 bp 1.0 55.1
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 548-558 bp 1.0 55.1
trnS-GCU-trnG-GCC_p3 GTTATCAGGGGAGTCGGAGA ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 616-626 bp 1.0 54.3
trnS-GCU-trnG-GCC_p4 AGTTATCAGGGGAGTCGGAG ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 617-627 bp 1.0 54.2
trnS-GCU-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 551-561 bp 1.0 54.1