Hotspot

psbD-psaI

LSC · 100605-102276 · 1672 bp

Back to Vriesea

Pi 0.0032

MarkerSeek score 61.20

Primer available yes

Label psbD-psaI

Feature Metadata

label_name: psbD-psaI
feature_type: igs
region: LSC
length_bp: 1672
variable_sites: 10
pi: 0.0032
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-psaI_p1	GGACCAGCCTACAAAAACGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1839-1874 bp	1.0	79.7
psbD-psaI_p2	GGACCAGCCTACAAAAACGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	1845-1880 bp	1.0	79.1
psbD-psaI_p3	ACAAAAACGAAACGGTCCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.2	58.0	1829-1864 bp	1.0	78.7
psbD-psaI_p4	ACAAAAACGAAACGGTCCCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.2	57.9	1835-1870 bp	1.0	78.1
psbD-psaI_p5	CAAAAACGAAACGGTCCCTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1828-1863 bp	1.0	77.5