Hotspot

accD-rbcL

LSC · 76196-76887 · 692 bp

Back to Vriesea

Pi 0.00145

MarkerSeek score 60.30

Primer available yes

Label accD-rbcL

Feature Metadata

label_name: accD-rbcL
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 2
pi: 0.00145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rbcL_p1	ATCACTTCCACTGGTATGGC	CTAGCAAATGGAGCCCTGAG	50.0	55.0	57.3	58.0	906-910 bp	1.0	74.5
accD-rbcL_p2	ATCACTTCCACTGGTATGGC	TGAAGCTAGCAAATGGAGCC	50.0	50.0	57.3	58.2	911-915 bp	1.0	72.8
accD-rbcL_p3	ATCACTTCCACTGGTATGGC	TCGAGTTCGAACCGGTAGAT	50.0	50.0	57.3	58.3	849-853 bp	1.0	72.8
accD-rbcL_p4	ATCACTTCCACTGGTATGGC	CCGTGAAGCTAGCAAATGGA	50.0	50.0	57.3	58.3	914-918 bp	1.0	72.7
accD-rbcL_p5	ATCACTTCCACTGGTATGGC	TCCGTGAAGCTAGCAAATGG	50.0	50.0	57.3	58.3	915-919 bp	1.0	72.7