Hotspot

psbM-trnD-GUC

LSC · 72444-73474 · 1031 bp

Back to Vriesea

Pi 0.00242

MarkerSeek score 60.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 5
pi: 0.00242
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1159-1161 bp	1.0	62.0
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1163-1165 bp	1.0	61.4
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	45.0	55.0	55.8	58.4	1165-1167 bp	1.0	59.2
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1166-1168 bp	1.0	58.9
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1142-1144 bp	1.0	58.7