Hotspot

matK-trnK-UUU

LSC · 45022-45844 · 823 bp

Back to Vriesea

Pi 0.00182

MarkerSeek score 60.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 3
pi: 0.00182
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAACGC	TTTGATAGGGTCGTGCAGTG	50.0	50.0	57.9	57.9	1037-1040 bp	1.0	78.6
matK-trnK-UUU_p2	CCATGATCATGAGCAAACGC	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.9	57.9	970-973 bp	1.0	78.5
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCCGAG	TTTGATAGGGTCGTGCAGTG	50.0	50.0	57.8	57.9	987-990 bp	1.0	78.3
matK-trnK-UUU_p4	TAGGAAGTGTTGTTGCCGAG	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.8	57.9	920-923 bp	1.0	78.2
matK-trnK-UUU_p5	CCATGATCATGAGCAAACGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	952-955 bp	1.0	78.1