Hotspot

rpl32-trnL-UAG

SSC · 175-927 · 753 bp

Back to Vriesea

Pi 0.00598

MarkerSeek score 49.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 753
variable_sites: 9
pi: 0.00598
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	837-840 bp	1.0	77.0
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	838-841 bp	1.0	77.0
rpl32-trnL-UAG_p3	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	840-843 bp	1.0	76.5
rpl32-trnL-UAG_p4	CACCGGGCATTCAAAAAGTT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	57.5	58.0	1423-1426 bp	1.0	76.2
rpl32-trnL-UAG_p5	CACCGGGCATTCAAAAAGTT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	57.5	58.0	1422-1425 bp	1.0	76.2