Hotspot

ycf1

SSC · 119586-124721 · 5136 bp

Back to Vittaria

Pi 0.473

MarkerSeek score 73.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5136
variable_sites: 1969
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGGGTTTTCTTGGCTTCGA	CCAACCACAGCTTTCTCACT	45.0	50.0	57.9	58.0	944 bp	0.5	59.3
ycf1_p2	TTGGGTTTTCTTGGCTTCGA	TCCAACCACAGCTTTCTCAC	45.0	50.0	57.9	58.0	945 bp	0.5	59.3
ycf1_p3	TTGGGTTTTCTTGGCTTCGA	TGCGTTCCAGAAGACGAAAA	45.0	45.0	57.9	58.1	649 bp	0.5	59.1
ycf1_p4	TTGGGTTTTCTTGGCTTCGA	TCCAGGAGTAGATGGCGATT	45.0	50.0	57.9	57.9	804 bp	0.5	58.8
ycf1_p5	TTGGGTTTTCTTGGCTTCGA	TTCCAGGAGTAGATGGCGAT	45.0	50.0	57.9	57.9	805 bp	0.5	58.8