Hotspot

rps15

SSC · 119115-119384 · 270 bp

Back to Vittaria

Pi 0.516
MarkerSeek score 77.20
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
270
variable_sites
131
pi
0.516
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 CGACAATAAGCCGGAGAACA CGTATCTCCATCACGTTCCC 50.0 55.0 58.0 57.9 863 bp 0.5 58.9
rps15_p2 CGACAATAAGCCGGAGAACA GTATCTCCATCACGTTCCCG 50.0 55.0 58.0 57.9 862 bp 0.5 58.9
rps15_p3 ACGACAATAAGCCGGAGAAC CGTATCTCCATCACGTTCCC 50.0 55.0 58.0 57.9 864 bp 0.5 58.9
rps15_p4 ACGACAATAAGCCGGAGAAC GTATCTCCATCACGTTCCCG 50.0 55.0 58.0 57.9 863 bp 0.5 58.9
rps15_p5 CGACAATAAGCCGGAGAACA TTCCCGGACCAATTGGAATC 50.0 50.0 58.0 57.9 848 bp 0.5 58.9