Hotspot

ndhI

SSC · 115215-115751 · 537 bp

Back to Vittaria

Pi 0.48

MarkerSeek score 78.90

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 537
variable_sites: 214
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TACCGGTTTATCAGCAACGG	GCAGAATGTGAAAGGTTGCC	50.0	50.0	58.0	57.9	1537 bp	0.5	59.3
ndhI_p2	TACCGGTTTATCAGCAACGG	GGCAGAATGTGAAAGGTTGC	50.0	50.0	58.0	57.9	1538 bp	0.5	59.3
ndhI_p3	TACCGGTTTATCAGCAACGG	ATTGCTAGACCTCGGATGGA	50.0	50.0	58.0	57.9	1190 bp	0.5	59.2
ndhI_p4	TACCGGTTTATCAGCAACGG	AATCTGTGGCGACAACCAAT	50.0	45.0	58.0	58.1	1588 bp	0.5	59.2
ndhI_p5	TACCGGTTTATCAGCAACGG	ATTCGGGGATACGATTTGGC	50.0	50.0	58.0	58.1	1464 bp	0.5	59.0