Hotspot

ndhH

SSC · 117802-118986 · 1185 bp

Back to Vittaria

Pi 0.483

MarkerSeek score 80.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1185
variable_sites: 483
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAACCATACCCTGCCATGAG	TCCTCGGGGAAATAGGTTGT	55.0	50.0	58.0	58.0	1696 bp	0.5	59.5
ndhH_p2	GAACCATACCCTGCCATGAG	GATTCACCGCTTCCCAGATT	55.0	50.0	58.0	58.0	1990 bp	0.5	59.4
ndhH_p3	GCTGGTCCGGCACTAAATAA	TCCTCGGGGAAATAGGTTGT	50.0	50.0	58.0	58.0	1549 bp	0.5	59.4
ndhH_p4	TGCTTCGGAAAGAGTTGCTT	TCCTCGGGGAAATAGGTTGT	45.0	50.0	58.0	58.0	1319 bp	0.5	59.4
ndhH_p5	TTGCTTCGGAAAGAGTTGCT	TCCTCGGGGAAATAGGTTGT	45.0	50.0	58.0	58.0	1320 bp	0.5	59.4