Hotspot

ndhE

SSC · 114063-114362 · 300 bp

Back to Vittaria

Pi 0.515

MarkerSeek score 73.50

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 300
variable_sites: 153
pi: 0.515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CCACGCAATCTTCCGTTCTA	CCGTTGCTGATAAACCGGTA	50.0	50.0	58.0	58.0	1202 bp	0.5	59.9
ndhE_p2	CAAATAGACACGGACGCTCA	CCGTTGCTGATAAACCGGTA	50.0	50.0	58.0	58.0	1261 bp	0.5	59.8
ndhE_p3	CTCGGGTATGAGCTGAGGTA	CCGTTGCTGATAAACCGGTA	55.0	50.0	58.0	58.0	1358 bp	0.5	59.8
ndhE_p4	CAATCTTCCGTTCTAGGGGC	CCGTTGCTGATAAACCGGTA	55.0	50.0	58.0	58.0	1197 bp	0.5	59.5
ndhE_p5	GCAATCTTCCGTTCTAGGGG	CCGTTGCTGATAAACCGGTA	55.0	50.0	58.0	58.0	1198 bp	0.5	59.5