Hotspot

chlL

SSC · 126540-127418 · 879 bp

Back to Vittaria

Pi 0.416

MarkerSeek score 79.50

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: SSC
length_bp: 879
variable_sites: 292
pi: 0.416
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	AGATCGTTACTCCGAGAGCA	TTCGAATAGAAAAGCCCCCG	50.0	50.0	58.0	58.0	1443 bp	0.5	59.4
chlL_p2	CCATTGCATAGCGAGGTTCT	TTCGAATAGAAAAGCCCCCG	50.0	50.0	58.0	58.0	1467 bp	0.5	59.4
chlL_p3	AGATCGTTACTCCGAGAGCA	CTCCCAAGAGGCGTAGTAGA	50.0	55.0	58.0	57.9	1308 bp	0.5	59.2
chlL_p4	CCATTGCATAGCGAGGTTCT	CTCCCAAGAGGCGTAGTAGA	50.0	55.0	58.0	57.9	1332 bp	0.5	59.2
chlL_p5	AGATCGTTACTCCGAGAGCA	GATCAACCTCACAATCCCCC	50.0	55.0	58.0	57.9	1376 bp	0.5	59.2