Hotspot

ccsA

SSC · 110539-111492 · 954 bp

Back to Vittaria

Pi 0.487

MarkerSeek score 81.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 954
variable_sites: 405
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAGCATCTCGGTTCGAATCC	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.1	58.1	1867 bp	0.5	59.0
ccsA_p2	ATAGGTAGACACGCTGCTCT	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	1902 bp	0.5	58.9
ccsA_p3	AAGCATCTCGGTTCGAATCC	CCAGGAATGAGTGGCTTTGT	50.0	50.0	58.1	58.1	1859 bp	0.5	58.9
ccsA_p4	ATAGGTAGACACGCTGCTCT	CCAGGAATGAGTGGCTTTGT	50.0	50.0	57.9	58.1	1894 bp	0.5	58.8
ccsA_p5	CAAGATGGGACGCTACCAAA	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	2199 bp	0.5	58.6