Hotspot

rrn16-trnR-ACG

IRa · 147963-148899 · 937 bp

Back to Vittaria

Pi 0.0365
MarkerSeek score 78.00
Primer available yes
Label rrn16-trnR-ACG

Feature Metadata

label_name
rrn16-trnR-ACG
feature_type
igs
region
IRa
length_bp
937
variable_sites
31
pi
0.0365
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnR-ACG_p1 CCAGGATCAAACTCTCCACG GTGTGGTACGAAACTCCGTT 55.0 50.0 58.0 58.1 978-1065 bp 1.0 85.2
rrn16-trnR-ACG_p2 CCAGGATCAAACTCTCCACG AAGTGTGGTACGAAACTCCG 55.0 50.0 58.0 57.9 980-1067 bp 1.0 85.1
rrn16-trnR-ACG_p3 CCACTGGAAACACCACTAGG GTGTGGTACGAAACTCCGTT 55.0 50.0 57.8 58.1 1052-1139 bp 1.0 83.6
rrn16-trnR-ACG_p4 CCACTGGAAACACCACTAGG AAGTGTGGTACGAAACTCCG 55.0 50.0 57.8 57.9 1054-1141 bp 1.0 83.6
rrn16-trnR-ACG_p5 GGAAACACCACTAGGATGCC GTGTGGTACGAAACTCCGTT 55.0 50.0 58.2 58.1 1047-1134 bp 1.0 83.1