Hotspot

chlN-chlL

SSC · 126464-126539 · 76 bp

Back to Vittaria

Pi 0.431

MarkerSeek score 77.30

Primer available yes

Label chlN-chlL

Feature Metadata

label_name: chlN-chlL
feature_type: igs
region: SSC
length_bp: 76
variable_sites: 31
pi: 0.431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-chlL_p1	TGAGAAGGTAACCAGCCTGA	GGTTATGGTGGCGTAGACTG	50.0	55.0	58.0	58.1	1416 bp	0.5	59.3
chlN-chlL_p2	AGATCGTTACTCCGAGAGCA	GGTTATGGTGGCGTAGACTG	50.0	55.0	58.0	58.1	899 bp	0.5	59.2
chlN-chlL_p3	CCATTGCATAGCGAGGTTCT	GGTTATGGTGGCGTAGACTG	50.0	55.0	58.0	58.1	923 bp	0.5	59.2
chlN-chlL_p4	TGAGAAGGTAACCAGCCTGA	CTGTGTAGAAGCTGGTGGAC	50.0	55.0	58.0	57.9	1399 bp	0.5	59.1
chlN-chlL_p5	TGAGAAGGTAACCAGCCTGA	GACTGTGTAGAAGCTGGTGG	50.0	55.0	58.0	57.9	1401 bp	0.5	59.1