Hotspot

ccsA-ndhD

SSC · 111493-111852 · 360 bp

Back to Vittaria

Pi 0.435

MarkerSeek score 77.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 360
variable_sites: 141
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTAAGCGCGACGTTGATTA	TTGCATTGCCAGGAATGAGT	45.0	45.0	58.0	58.1	1254 bp	0.5	59.3
ccsA-ndhD_p2	TGTAAGCGCGACGTTGATTA	TAAAATGGGCGGATATGGGC	45.0	50.0	58.0	58.1	1649 bp	0.5	59.2
ccsA-ndhD_p3	TGTAAGCGCGACGTTGATTA	CCAGGAATGAGTGGCTTTGT	45.0	50.0	58.0	58.1	1246 bp	0.5	59.2
ccsA-ndhD_p4	CTTTTTGGAGTTGGGACCCA	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	919 bp	0.5	58.8
ccsA-ndhD_p5	TTGGAGTTGGGACCCAAAAG	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	915 bp	0.5	58.8