Hotspot

trnR-ACG-rrn16

IRb · 84963-85899 · 937 bp

Back to Vittaria

Pi 0.0365
MarkerSeek score 78.00
Primer available yes
Label trnR-ACG-rrn16

Feature Metadata

label_name
trnR-ACG-rrn16
feature_type
igs
region
IRb
length_bp
937
variable_sites
31
pi
0.0365
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn16_p1 GTGTGGTACGAAACTCCGTT CCAGGATCAAACTCTCCACG 50.0 55.0 58.1 58.0 978-1065 bp 1.0 85.2
trnR-ACG-rrn16_p2 AAGTGTGGTACGAAACTCCG CCAGGATCAAACTCTCCACG 50.0 55.0 57.9 58.0 980-1067 bp 1.0 85.1
trnR-ACG-rrn16_p3 GTGTGGTACGAAACTCCGTT CCACTGGAAACACCACTAGG 50.0 55.0 58.1 57.8 1052-1139 bp 1.0 83.6
trnR-ACG-rrn16_p4 AAGTGTGGTACGAAACTCCG CCACTGGAAACACCACTAGG 50.0 55.0 57.9 57.8 1054-1141 bp 1.0 83.6
trnR-ACG-rrn16_p5 GTGTGGTACGAAACTCCGTT GGAAACACCACTAGGATGCC 50.0 55.0 58.1 58.2 1047-1134 bp 1.0 83.1