Hotspot

rpoB-trnD-GUC

LSC · 26676-29742 · 3067 bp

Back to Vittaria

Pi 0.384

MarkerSeek score 81.60

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 3067
variable_sites: 1117
pi: 0.384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	GGTGACACCTACCACGATTC	GGCCTTTCTGCGGCTATTAT	55.0	50.0	58.0	58.1	817 bp	0.5	59.1
rpoB-trnD-GUC_p2	GGTGACACCTACCACGATTC	CGCTCCCAGATATCCCATTG	55.0	55.0	58.0	57.9	620 bp	0.5	59.1
rpoB-trnD-GUC_p3	ACCATTCGAGGATACCGGAT	GGCCTTTCTGCGGCTATTAT	50.0	50.0	58.0	58.1	584 bp	0.5	59.1
rpoB-trnD-GUC_p4	ACCATTCGAGGATACCGGAT	CGCTCCCAGATATCCCATTG	50.0	55.0	58.0	57.9	387 bp	0.5	59.1
rpoB-trnD-GUC_p5	GGTGACACCTACCACGATTC	AAGAGGTTTGTCTCAGGGGA	55.0	50.0	58.0	57.9	570 bp	0.5	59.0