Hotspot

rps15-ycf1

SSC · 129736-130042 · 307 bp

Back to Vitis

Pi 0.00386
MarkerSeek score 49.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
307
variable_sites
16
pi
0.00386
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGATCCCCTATTTGCT TCCACGATTGAAAATTCGTTGA 42.9 36.4 56.7 56.9 385-399 bp 1.0 45.5
rps15-ycf1_p2 TCAACAGATCCCCTATTTGCT GCTTGTATGAATCGCTATTGGT 42.9 40.9 56.7 56.9 456-470 bp 1.0 44.7
rps15-ycf1_p3 TCAACAGATCCCCTATTTGCT AGCTTGTATGAATCGCTATTGGT 42.9 39.1 56.7 58.3 457-471 bp 1.0 44.7
rps15-ycf1_p4 TCAACAGATCCCCTATTTGCT AGCTTGTATGAATCGCTATTGG 42.9 40.9 56.7 56.7 457-471 bp 1.0 44.7
rps15-ycf1_p5 TCAACAGATCCCCTATTTGCTT GCTTGTATGAATCGCTATTGGT 40.9 40.9 57.4 56.9 456-470 bp 1.0 44.7