Hotspot

psbB-psbT

LSC · 78907-79093 · 187 bp

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Pi 0.000556
MarkerSeek score 28.10
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
187
variable_sites
2
pi
0.000556
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 GCTGGTATTGACCCGGATTT GAACTTTAGGCGGTTCTCGA 50.0 50.0 57.9 57.6 365 bp 1.0 77.4
psbB-psbT_p2 GCTGGTATTGACCCGGATTT TGGAACTTTAGGCGGTTCTC 50.0 50.0 57.9 57.5 367 bp 1.0 76.5
psbB-psbT_p3 CCGGATTTGGATGCTCAAGT GAACTTTAGGCGGTTCTCGA 50.0 50.0 58.2 57.6 353 bp 1.0 75.8
psbB-psbT_p4 TCAGAGACGTTTTTGCTGGT GAACTTTAGGCGGTTCTCGA 45.0 50.0 57.7 57.6 379 bp 1.0 75.2
psbB-psbT_p5 GCTGGTATTGACCCGGATTT GGAACTTTAGGCGGTTCTCG 50.0 55.0 57.9 58.6 366 bp 1.0 75.1