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psbE-petL

LSC · 69434-70739 · 1306 bp

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Pi 0.00346
MarkerSeek score 55.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1306
variable_sites
32
pi
0.00346
unique_haplotype_count
36
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.8 58.0 1368-1575 bp 1.0 81.7
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.8 58.0 1370-1577 bp 1.0 81.7
psbE-petL_p3 ACGAATAACCAACCCGCAAT GCCGCTAGTAGAAAACCGAA 45.0 50.0 58.2 58.0 1454-1661 bp 1.0 81.6
psbE-petL_p4 CAACCCGCAATGAATAGGGA GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.9 58.0 1445-1637 bp 0.982456 81.6
psbE-petL_p5 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.9 58.0 1447-1639 bp 0.982456 81.6