Hotspot

accD-psaI

LSC · 63010-63764 · 755 bp

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Pi 0.00507
MarkerSeek score 55.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
755
variable_sites
33
pi
0.00507
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTTCACGCTTTCTTTCCTT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.9 58.0 858-873 bp 1.0 75.6
accD-psaI_p2 GCTTCACGCTTTCTTTCCTTT CCATTGCAATTGCCGGAAAT 42.9 45.0 57.6 58.0 858-873 bp 1.0 73.3
accD-psaI_p3 GCTTCACGCTTTCTTTCCTT AACGAAGCCATTGCAATTGC 45.0 45.0 56.9 58.6 865-880 bp 1.0 71.2
accD-psaI_p4 AAAGGGTGTTCTGAGTGAGT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.3 58.0 885-900 bp 1.0 70.4
accD-psaI_p5 AAGGGTGTTCTGAGTGAGTT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.3 58.0 884-899 bp 1.0 70.4