Hotspot

psbM-trnD-GUC

LSC · 31993-33115 · 1123 bp

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Pi 0.00273
MarkerSeek score 53.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1123
variable_sites
50
pi
0.00273
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TGGGCTATGGCTATACACCT 45.0 50.0 55.8 57.6 1263-1277 bp 1.0 63.5
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1224-1238 bp 1.0 63.1
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC GTGGGCTATGGCTATACACC 45.0 55.0 55.8 57.5 1264-1278 bp 1.0 63.0
psbM-trnD-GUC_p4 AGGAACTAGAATGAACAGTGCA TGGGCTATGGCTATACACCT 40.9 50.0 57.4 57.6 1270-1284 bp 1.0 60.3
psbM-trnD-GUC_p5 AGGAACTAGAATGAACAGTGCA GTGGGCTATGGCTATACACC 40.9 55.0 57.4 57.5 1271-1285 bp 1.0 59.8