Hotspot

trnS-GCU-trnG-GCC

LSC · 9542-10548 · 1007 bp

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Pi 0.00302
MarkerSeek score 52.40
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1007
variable_sites
49
pi
0.00302
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 ATTAGCAATCCGCCGCTTTA GGGGGTTAATAGAACGAATCACA 45.0 43.5 58.0 58.2 1088-1112 bp 1.0 58.4
trnS-GCU-trnG-GCC_p2 ACGGAAAGAGAGGGATTCGA GGGGGTTAATAGAACGAATCACA 50.0 43.5 58.2 58.2 1137-1161 bp 1.0 57.3
trnS-GCU-trnG-GCC_p3 ATTAGCAATCCGCCGCTTTA GGGGGTTAATAGAACGAATCAC 45.0 45.5 58.0 56.6 1088-1112 bp 1.0 56.9
trnS-GCU-trnG-GCC_p4 TGGAAACGGAAAGAGAGGGA GGGGGTTAATAGAACGAATCACA 50.0 43.5 58.3 58.2 1142-1166 bp 1.0 56.3
trnS-GCU-trnG-GCC_p5 ACGGAAAGAGAGGGATTCGA GGGGGTTAATAGAACGAATCAC 50.0 45.5 58.2 56.6 1137-1161 bp 1.0 55.8