Hotspot

ycf1

SSC · 124336-129882 · 5547 bp

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Pi 0.0127
MarkerSeek score 45.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5547
variable_sites
233
pi
0.0127
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CTATCCCAGGCCTCTGCTAT AAGGTACGACCCTTTCCTGA 55.0 50.0 58.1 58.0 918-930 bp 0.769231 75.0
ycf1_p2 TATCCCAGGCCTCTGCTATC AAGGTACGACCCTTTCCTGA 55.0 50.0 58.1 58.0 917-929 bp 0.769231 74.9
ycf1_p3 GCGCAAATGGAATGCTATCC AAGGTACGACCCTTTCCTGA 50.0 50.0 57.9 58.0 932-944 bp 0.769231 74.8
ycf1_p4 CAAATCCTCAGACCACTCGG AAGGTACGACCCTTTCCTGA 55.0 50.0 58.0 58.0 1075 bp 0.615385 64.6
ycf1_p5 CAAATCCTCAGACCACTCGG AGAAGGATATCCCCGCCTAC 55.0 55.0 58.0 58.1 1284 bp 0.615385 64.3