Hotspot

ndhF

SSC · 110859-113105 · 2247 bp

Pi 0.0119

MarkerSeek score 47.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 89
pi: 0.0119
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCCGGTGA	50.0	50.0	58.0	58.0	2522-2529 bp	1.0	82.6
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	2521-2528 bp	1.0	82.6
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAAGAGGTAAGGTCCGGTGA	50.0	50.0	58.0	58.0	2514-2521 bp	1.0	82.5
ndhF_p4	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	2513-2520 bp	1.0	82.5
ndhF_p5	AAGAAGATCCTCCCCCTTCC	AAGAGGTAAGGTCCGGTGAA	55.0	50.0	58.1	58.0	2498-2505 bp	1.0	82.1