Hotspot

rps15-ycf1

SSC · 123916-124335 · 420 bp

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Pi 0.034
MarkerSeek score 63.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
420
variable_sites
41
pi
0.034
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCGTTGACGTTTTCCCAA AGCAGCCAAGTTTCACATGA 45.0 45.0 58.0 58.0 782-833 bp 1.0 88.8
rps15-ycf1_p2 AGTCGTTGACGTTTTCCCAA GCAGCCAAGTTTCACATGAC 45.0 50.0 58.0 57.9 781-832 bp 1.0 88.5
rps15-ycf1_p3 CCGAGTCATTCTTCCATGCA AGCAGCCAAGTTTCACATGA 50.0 45.0 58.0 58.0 956-1007 bp 1.0 88.4
rps15-ycf1_p4 AACACCATGCATTGATGGGT AGCAGCCAAGTTTCACATGA 45.0 45.0 58.1 58.0 1040-1091 bp 1.0 88.0
rps15-ycf1_p5 ACCATGCATTGATGGGTGTT AGCAGCCAAGTTTCACATGA 45.0 45.0 58.1 58.0 1037-1052 bp 0.615385 65.3