Hotspot

ccsA-ndhD

SSC · 115789-116054 · 266 bp

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Pi 0.0436
MarkerSeek score 67.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
266
variable_sites
36
pi
0.0436
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GTTGTGGCGTCTATGGGATT CAGTAGCTTTTCGATGGCCT 50.0 50.0 58.0 58.0 744-765 bp 0.923077 82.6
ccsA-ndhD_p2 CGGCCCTTTTATGTGGATCA CAGTAGCTTTTCGATGGCCT 50.0 50.0 57.9 58.0 1166-1187 bp 0.923077 81.6
ccsA-ndhD_p3 GTTGTGGCGTCTATGGGATT TCAGTAGCTTTTCGATGGCC 50.0 50.0 58.0 58.0 745-766 bp 0.846154 79.3
ccsA-ndhD_p4 TTGGAGTTGGGACCCAAAAG TCAGTAGCTTTTCGATGGCC 50.0 50.0 57.9 58.0 851-872 bp 0.846154 78.5
ccsA-ndhD_p5 CGGCCCTTTTATGTGGATCA TCAGTAGCTTTTCGATGGCC 50.0 50.0 57.9 58.0 1167-1188 bp 0.846154 78.4