Hotspot

ndhF-trnL-UAG

SSC · 113106-114631 · 1526 bp

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Pi 0.0238
MarkerSeek score 68.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1526
variable_sites
111
pi
0.0238
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CTGGAAGTGGAACGAAAGGT TTTAGGAGATGACATGCCGC 50.0 50.0 57.7 58.0 1362-1683 bp 1.0 87.0
ndhF-trnL-UAG_p2 CTGGAAGTGGAACGAAAGGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 1307-1628 bp 1.0 86.6
ndhF-trnL-UAG_p3 CTGGAAGTGGAACGAAAGGT AGCAGCGTGTCTACCAATTT 50.0 45.0 57.7 57.8 1300-1621 bp 1.0 86.1
ndhF-trnL-UAG_p4 CTGGAAGTGGAACGAAAGGT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.7 57.8 1306-1627 bp 1.0 86.0
ndhF-trnL-UAG_p5 CTGGAAGTGGAACGAAAGGT CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.7 57.8 1305-1626 bp 1.0 86.0