Hotspot

rpl16-rps3

LSC · 82743-83798 · 1056 bp

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Pi 0.0126
MarkerSeek score 60.10
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1056
variable_sites
52
pi
0.0126
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 ACGGAATCTAGTTCTTTTGGGG TGGGGTATTGGGCATCAAAA 45.5 45.0 58.0 57.4 1056-1164 bp 1.0 66.3
rpl16-rps3_p2 CGGAATCTAGTTCTTTTGGGGT TGGGGTATTGGGCATCAAAA 45.5 45.0 58.0 57.4 1055-1163 bp 1.0 66.3
rpl16-rps3_p3 ACGGAATCTAGTTCTTTTGGGG ATGGGGTATTGGGCATCAAA 45.5 45.0 58.0 57.1 1057-1165 bp 1.0 64.4
rpl16-rps3_p4 CGGAATCTAGTTCTTTTGGGGT ATGGGGTATTGGGCATCAAA 45.5 45.0 58.0 57.1 1056-1164 bp 1.0 64.4
rpl16-rps3_p5 CGGAATCTAGTTCTTTTGGGGT TGGGGTATTGGGCATCAAAAT 45.5 42.9 58.0 57.9 1055-1163 bp 1.0 62.3