Hotspot

rps8-rpl14

LSC · 81654-81836 · 183 bp

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Pi 0.0266
MarkerSeek score 57.10
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
183
variable_sites
13
pi
0.0266
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TGCTATGCGAACAACTCCTT GTTTCATTAGCTCCCGAGGT 45.0 50.0 57.5 57.3 288-295 bp 1.0 75.6
rps8-rpl14_p2 ATGCTATGCGAACAACTCCT GTTTCATTAGCTCCCGAGGT 45.0 50.0 57.3 57.3 289-296 bp 1.0 73.8
rps8-rpl14_p3 TGCTATGCGAACAACTCCTT AGTTTCATTAGCTCCCGAGG 45.0 50.0 57.5 57.0 289-296 bp 1.0 73.2
rps8-rpl14_p4 ATGCTATGCGAACAACTCCT AGTTTCATTAGCTCCCGAGG 45.0 50.0 57.3 57.0 290-297 bp 1.0 71.4
rps8-rpl14_p5 ATGCTATGCGAACAACTCCTT GTTTCATTAGCTCCCGAGGT 42.9 50.0 58.0 57.3 289-296 bp 1.0 71.3