Hotspot

rpl20-rps12

LSC · 70087-70917 · 831 bp

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Pi 0.0104
MarkerSeek score 57.00
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
831
variable_sites
27
pi
0.0104
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACCCTCGAAAACTTGATGCA ACAGCCCATTCGAAATGTCA 45.0 45.0 57.7 57.8 985-998 bp 1.0 79.8
rpl20-rps12_p2 CGGGCTATATATCCGCGTTT ACAGCCCATTCGAAATGTCA 50.0 45.0 57.7 57.8 938-951 bp 1.0 79.8
rpl20-rps12_p3 TCTCCGGGCTATATATCCGC ACAGCCCATTCGAAATGTCA 55.0 45.0 58.3 57.8 942-955 bp 1.0 79.6
rpl20-rps12_p4 ACCCTCGAAAACTTGATGCA ACAAGACAGCCCATTCGAAA 45.0 45.0 57.7 57.7 990-1003 bp 1.0 79.2
rpl20-rps12_p5 CGGGCTATATATCCGCGTTT ACAAGACAGCCCATTCGAAA 50.0 45.0 57.7 57.7 943-956 bp 1.0 79.2