Hotspot

ndhC-trnM-CAU

LSC · 51464-53337 · 1874 bp

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Pi 0.00803
MarkerSeek score 56.20
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1874
variable_sites
56
pi
0.00803
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 TGCGAAAATAGGAATAAGACTTGA CCCGCCGTATGAAAGCAATA 33.3 50.0 56.5 58.4 1964-1990 bp 1.0 44.2
ndhC-trnM-CAU_p2 TGCGAAAATAGGAATAAGACTTGA CCGCCGTATGAAAGCAATAC 33.3 50.0 56.5 57.3 1963-1989 bp 1.0 44.2
ndhC-trnM-CAU_p3 TGCGAAAATAGGAATAAGACTTGA TTTGAACCAATGACTCCCGC 33.3 50.0 56.5 58.8 1979-2005 bp 1.0 44.2
ndhC-trnM-CAU_p4 TGCGAAAATAGGAATAAGACTTG CCCGCCGTATGAAAGCAATA 34.8 50.0 55.1 58.4 1964-1990 bp 1.0 44.2
ndhC-trnM-CAU_p5 TGCGAAAATAGGAATAAGACTTGAT CCCGCCGTATGAAAGCAATA 32.0 50.0 56.9 58.4 1964-1990 bp 1.0 44.2