Hotspot

petN-psbM

LSC · 29795-30799 · 1005 bp

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Pi 0.0157
MarkerSeek score 55.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1005
variable_sites
49
pi
0.0157
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA GACGACGGGTTGATTTGAGA 50.0 50.0 58.0 57.9 1303-1304 bp 0.615385 64.5
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT GACGACGGGTTGATTTGAGA 50.0 50.0 58.0 57.9 1301-1302 bp 0.615385 64.5
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA ACGACGGGTTGATTTGAGAG 50.0 50.0 58.0 57.9 1302-1303 bp 0.615385 64.5
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT ACGACGGGTTGATTTGAGAG 50.0 50.0 58.0 57.9 1300-1301 bp 0.615385 64.5
petN-psbM_p5 TTTGGATTGGAAAGGGGTGG GACGACGGGTTGATTTGAGA 50.0 50.0 58.0 57.9 1910-1938 bp 0.615385 64.3