Hotspot

atpH-atpI

LSC · 14246-15279 · 1034 bp

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Pi 0.0135
MarkerSeek score 57.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1034
variable_sites
40
pi
0.0135
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.1 57.9 1056-1120 bp 1.0 85.0
atpH-atpI_p2 AACGGAAGCAGCAGAAATCA CCCGTCATGTTCCTTGGATT 45.0 50.0 58.1 57.9 1103-1167 bp 1.0 84.9
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA TTTTGCAACTTTAGCCGCAG 45.0 45.0 57.9 57.9 1074-1138 bp 1.0 84.7
atpH-atpI_p4 AGCCAATCCAGCAGCAATAA CCCGTCATGTTCCTTGGATT 45.0 50.0 57.9 57.9 1121-1185 bp 1.0 84.6
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC CCCGTCATGTTCCTTGGATT 50.0 50.0 57.8 57.9 1120-1184 bp 1.0 84.0