Hotspot

rps16-trnQ-UUG

LSC · 6364-7604 · 1241 bp

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Pi 0.015
MarkerSeek score 60.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1241
variable_sites
60
pi
0.015
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAAATTGAAATGGGGCGTGG 50.0 50.0 57.9 58.0 673-1390 bp 1.0 87.2
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 615-1332 bp 1.0 86.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 656-1373 bp 1.0 86.8
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 598-1315 bp 1.0 86.5
rps16-trnQ-UUG_p5 CAACGGATCGTGTCCTTCAA GAAATTGAAATGGGGCGTGG 50.0 50.0 58.2 58.0 674-1391 bp 1.0 86.2