Hotspot

trnK-UUU-rps16

LSC · 4302-5204 · 903 bp

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Pi 0.0164
MarkerSeek score 59.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
903
variable_sites
46
pi
0.0164
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.90
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGAAAGCGGAAGTTTTTAAGGA 50.0 36.4 58.2 56.2 895-1032 bp 1.0 56.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGAAAGCGGAAGTTTTTAAGGA 50.0 36.4 58.2 56.2 894-1031 bp 1.0 56.1
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAGCGGAAGTTTTTAAGGAACT 50.0 34.8 58.2 57.2 892-1029 bp 1.0 55.8
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAAGCGGAAGTTTTTAAGGAACT 50.0 34.8 58.2 57.2 893-1030 bp 1.0 55.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGCGGAAGTTTTTAAGGAAC 50.0 40.0 58.2 54.1 890-1027 bp 1.0 55.5