Hotspot

APQ78_gp033-APQ78_gp031

Genome · 59374-60173 · 800 bp

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Pi 0.0488
MarkerSeek score 84.50
Primer available yes
Label APQ78_gp033-APQ78_gp031

Feature Metadata

label_name
APQ78_gp033-APQ78_gp031
feature_type
igs
region
Genome
length_bp
800
variable_sites
85
pi
0.0488
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp033-APQ78_gp031_p1 TCGCCGTCTACGAGCTATAT CCTGGAAGCGGAAGAACTAC 50.0 55.0 57.9 58.0 1235-1287 bp 0.833333 82.6
APQ78_gp033-APQ78_gp031_p2 TCGCCGTCTACGAGCTATAT TGCCTGTACGGTTGATGATG 50.0 50.0 57.9 58.0 1371-1452 bp 0.833333 82.6
APQ78_gp033-APQ78_gp031_p3 AGGAGGATTCCCTATTCCCG TGCCTGTACGGTTGATGATG 55.0 50.0 58.0 58.0 1609-1639 bp 0.666667 73.1
APQ78_gp033-APQ78_gp031_p4 AGGAGGATTCCCTATTCCCG CCTGGAAGCGGAAGAACTAC 55.0 55.0 58.0 58.0 1457-1474 bp 0.666667 72.7
APQ78_gp033-APQ78_gp031_p5 AGGAGGATTCCCTATTCCCG TCCTGGAAGCGGAAGAACTA 55.0 50.0 58.0 58.1 1458-1475 bp 0.666667 72.3