Hotspot

APQ78_gp038-APQ78_gp037

Genome · 55636-56496 · 861 bp

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Pi 0.0862
MarkerSeek score 84.00
Primer available yes
Label APQ78_gp038-APQ78_gp037

Feature Metadata

label_name
APQ78_gp038-APQ78_gp037
feature_type
igs
region
Genome
length_bp
861
variable_sites
161
pi
0.0862
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp038-APQ78_gp037_p1 ACGGTAGGTACAGCTAGTCC AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.0 58.0 1662-1777 bp 1.0 91.5
APQ78_gp038-APQ78_gp037_p2 GGTAGGTACAGCTAGTCCGT AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.0 58.0 1660-1775 bp 1.0 91.5
APQ78_gp038-APQ78_gp037_p3 ATATTCGTTTGGTCGAGGGC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 1490-1605 bp 1.0 91.5
APQ78_gp038-APQ78_gp037_p4 TCCTTGTCGGCTCTCTGTAA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 1511-1626 bp 1.0 91.2
APQ78_gp038-APQ78_gp037_p5 ATGGAATTCCTTGTCGGCTC AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 1595-1633 bp 0.666667 76.6