Hotspot

APQ78_gt011-APQ78_gp055

Genome · 32284-32716 · 433 bp

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Pi 0.14
MarkerSeek score 81.90
Primer available yes
Label APQ78_gt011-APQ78_gp055

Feature Metadata

label_name
APQ78_gt011-APQ78_gp055
feature_type
igs
region
Genome
length_bp
433
variable_sites
131
pi
0.14
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gt011-APQ78_gp055_p1 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 412-509 bp 1.0 83.6
APQ78_gt011-APQ78_gp055_p2 CTATCAACCACTCGGCCATC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 401-498 bp 1.0 83.0
APQ78_gt011-APQ78_gp055_p3 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGAA 55.0 38.1 58.1 57.7 412-509 bp 1.0 81.4
APQ78_gt011-APQ78_gp055_p4 CAAGACCGGAGCTATCAACC AAACAGCCAATTGGAAAGCA 55.0 40.0 58.1 56.7 407-504 bp 1.0 81.1
APQ78_gt011-APQ78_gp055_p5 CAAGACCGGAGCTATCAACC AACAGCCAATTGGAAAGCAA 55.0 40.0 58.1 56.7 406-503 bp 1.0 81.1